Journal: The American Journal of Pathology

Article Title: Critical Role of the CXCL10/C-X-C Chemokine Receptor 3 Axis in Promoting Leukocyte Recruitment and Neuronal Injury during Traumatic Optic Neuropathy Induced by Optic Nerve Crush

doi: 10.1016/j.ajpath.2016.10.009

Figure Lengend Snippet: CXCL10/CXCR3 pathway is activated in TON. Wild-type mice were subjected to traumatic optic neuropathy (TON). A: Quantitative PCR analysis of CXCL10 mRNA expression in noninjured retinas [control (Con)] or injured retinas at 3, 6, 12, and 24 hours after TON. B: Normal and TON-performed eyes were collected at 6 hours after TON. CXCL10 mRNA localization was assessed in retinal frozen sections by fluorescence in situ hybridization with RNAscope Fluorescent Multiplex Kit. Green fluorescent signal reflects CXCL10 mRNA expression, and DAPI (blue) stains nuclei. Arrows indicate CXCL10-expressing retinal cells in the ganglion cell layer (GCL). C: Enzyme-linked immunosorbent assay analysis of CXCL10 protein in control or TON-performed retinas at 6 hours after TON. D: Quantitative PCR analysis of CXCR3 mRNA expression in control or injured retinas at 3, 6, 12, and 24 hours after TON. E: Representative images of CXCR3 immunostaining in retinal frozen sections from control and TON-performed eyes at 24 hours after TON. Fluorescent signal (red) reflects CXCR3 staining. n = 4 to 5 mice (E). ∗P < 0.05, ∗∗P < 0.01 versus control. Scale bars = 50 μm (B and E). INL, inner nuclear layer; ONL, outer nuclear layer.

Article Snippet: Fluorescence in Situ Hybridization Retinal frozen sections were fixed in 4% paraformaldehyde, incubated with mouse CXCL10 probe (Advanced Cell Diagnostics, Hayward, CA), and sequentially hybridized to a cascade of signal amplification reagents from RNAscope Fluorescent Multiplex detection kit (Advanced Cell Diagnostics), according to the manufacturer's instructions.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Control, Fluorescence, In Situ Hybridization, RNAscope, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Immunostaining, Staining

Journal: Developmental Cell

Article Title: Subcellular mRNA Localization Regulates Ribosome Biogenesis in Migrating Cells

doi: 10.1016/j.devcel.2020.10.006

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Article Snippet: SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) , Cell Signaling Technologies , Cat#8114P.

Techniques: Virus, Cloning, Recombinant, Western Blot, Staining, Plasmid Preparation, RNAscope, Multiplex Assay, Control, CRISPR, Modification, Transfection, Fractionation, Peptide Fractionation, Sequencing, Cell Viability Assay, Viability Assay, RNA HS Assay, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Quantitative Proteomics, Multiplex sample analysis, Generated, Stable Transfection, Expressing, Negative Control, Software, Membrane, Cell Culture

Journal: Cell reports

Article Title: Loss of neuronal Tet2 enhances hippocampal-dependent cognitive function

doi: 10.1016/j.celrep.2022.111612

Figure Lengend Snippet:

Article Snippet: Following amplification steps, mouse Tet2 transcripts were detected using TSA Plus Cy3 reagents (Akoya Biosciences, Cat# SKU NEL744001KT).

Techniques: Recombinant, Magnetic Beads, RNAscope, Multiplex Assay, Reverse Transcription, Sequencing, shRNA, Expressing, Plasmid Preparation, Software